Pinto A E, Fonseca I, Soares J
Departamento de Patologia Morfológica e Centro de Investigação de Patobiologia Molecular do Instituto Português de Oncologia de Francisco Gentil, Centro de Lisboa, Portugal.
Cancer. 1999 Jan 15;85(2):273-81.
The authors studied a series of 97 consecutive cases of salivary gland tumors to investigate the correlation between the biologic parameters DNA ploidy and S-phase fraction (SPF) and the presumptive behavior of the neoplasms, as well as their potential clinical utility.
Histopathologic classification and grading of the tumors were evaluated according to 1991 World Health Organization criteria. DNA analysis was performed by flow cytometry in fresh material after propidium iodide staining. Clinical data and follow-up information were obtained from the clinical charts.
All the 71 benign salivary tumors showed a DNA diploid pattern. Seven carcinomas (7.2%) exhibited DNA aneuploidy. Eleven (42.3%) of 26 malignant tumors were considered low grade carcinomas, all of them being DNA diploid. Of the remaining 15 tumors, classified as high grade carcinomas, 7 showed DNA aneuploidy. SPF values ranged from 0.6% to 27.7%. A statistically significant difference was found between the mean SPF values of benign and malignant tumors, diploid and aneuploid tumors, and low grade and high grade carcinomas. When a cutoff value of 3% was used to discriminate histopathologic subgroups with prognostic impact, a significant difference was found between benign and malignant salivary tumors, high grade and low grade carcinomas, and high grade and benign tumors (P < 0.001).
The data from this study confirm the low incidence of DNA aneuploidy in salivary gland tumors and suggest the potential utility of SPF estimation in evaluating the clinical behavior of these neoplasms.
作者研究了连续97例涎腺肿瘤病例，以探讨生物学参数DNA倍体和S期细胞分数（SPF）与肿瘤推测行为之间的相关性，以及它们潜在的临床应用价值。
根据1991年世界卫生组织标准对肿瘤进行组织病理学分类和分级。采用碘化丙啶染色后，通过流式细胞术对新鲜标本进行DNA分析。临床资料和随访信息从临床病历中获取。
所有71例涎腺良性肿瘤均显示DNA二倍体模式。7例癌（7.2%）表现为DNA非整倍体。26例恶性肿瘤中有11例（42.3%）被认为是低级别癌，均为DNA二倍体。其余15例被归类为高级别癌的肿瘤中，7例显示DNA非整倍体。SPF值范围为0.6%至27.7%。良性和恶性肿瘤、二倍体和非整倍体肿瘤以及低级别和高级别癌的平均SPF值之间存在统计学显著差异。当使用3%的临界值来区分具有预后影响的组织病理学亚组时，涎腺良性和恶性肿瘤、高级别和低级别癌以及高级别和良性肿瘤之间存在显著差异（P < 0.001）。
本研究数据证实涎腺肿瘤中DNA非整倍体的发生率较低，并表明SPF评估在评估这些肿瘤的临床行为方面具有潜在应用价值。
