Nadji M, Nassiri M, Fresno M, Terzian E, Morales A R
Department of Pathology, University of Miami, Jackson Memorial Medical Center, Florida 33101, USA.
Cancer. 1999 Jan 15;85(2):432-6. doi: 10.1002/(sici)1097-0142(19990115)85:2<432::aid-cncr22>3.0.co;2-j.
Expression of 67 kD laminin binding protein, 67LR, is reported to be associated with invasive and metastatic phenotypes in several types of human malignancies. In mammary carcinomas, however, the biologic role of 67LR has been less clear. The authors explored the potential biologic significance of expression of 67LR in 148 patients with axillary lymph node negative breast carcinoma.
Formalin fixed, paraffin embedded histologic sections were immunohistochemically evaluated for 67LR using monoclonal antibody MLuC5. The staining results were correlated with morphologic data as well as with estrogen receptor content and p53 product accumulation.
There were statistically significant correlations between positivity for 67LR and lower histologic grade (P = 0.003), lower nuclear grade (P = 0.002), positivity for estrogen receptor (P = 0.003), and lack of p53 abnormality (P < 0.001). Expression of 67LR had no independent effect on the disease free or overall survival of lymph node negative patients with breast carcinoma. Nevertheless, in the subgroup of 67LR positive patients, positivity for estrogen receptor was associated with significantly longer overall survival (P = 0.008).
The data from this study suggest that tissue expression of 67LR, as detected by antibody MLuC5, is associated with better differentiated, less aggressive forms of axillary lymph node negative breast carcinoma.
据报道，67kD层粘连蛋白结合蛋白（67LR）的表达与几种人类恶性肿瘤的侵袭和转移表型相关。然而，在乳腺癌中，67LR的生物学作用尚不清楚。作者探讨了148例腋窝淋巴结阴性乳腺癌患者中67LR表达的潜在生物学意义。
使用单克隆抗体MLuC5对福尔马林固定、石蜡包埋的组织学切片进行67LR的免疫组织化学评估。将染色结果与形态学数据以及雌激素受体含量和p53产物积累情况进行关联分析。
67LR阳性与较低的组织学分级（P = 0.003）、较低的核分级（P = 0.002）、雌激素受体阳性（P = 0.003）以及缺乏p53异常（P < 0.001）之间存在统计学显著相关性。67LR的表达对腋窝淋巴结阴性乳腺癌患者的无病生存期或总生存期没有独立影响。然而，在67LR阳性患者亚组中，雌激素受体阳性与显著更长的总生存期相关（P = 0.008）。
本研究数据表明，通过抗体MLuC5检测到的67LR组织表达与分化更好、侵袭性更小的腋窝淋巴结阴性乳腺癌形式相关。
