Lowe T M, Eddy S R
Department of Genetics, Washington University School of Medicine, 4566 Scott Avenue, St. Louis, MO 63110, USA.
Science. 1999 Feb 19;283(5405):1168-71. doi: 10.1126/science.283.5405.1168.
Small nucleolar RNAs (snoRNAs) are required for ribose 2'-O-methylation of eukaryotic ribosomal RNA. Many of the genes for this snoRNA family have remained unidentified in Saccharomyces cerevisiae, despite the availability of a complete genome sequence. Probabilistic modeling methods akin to those used in speech recognition and computational linguistics were used to computationally screen the yeast genome and identify 22 methylation guide snoRNAs, snR50 to snR71. Gene disruptions and other experimental characterization confirmed their methylation guide function. In total, 51 of the 55 ribose methylated sites in yeast ribosomal RNA were assigned to 41 different guide snoRNAs.
小核仁RNA（snoRNAs）是真核生物核糖体RNA核糖2'-O-甲基化所必需的。尽管酿酒酵母已有完整的基因组序列，但该snoRNA家族的许多基因仍未被鉴定出来。类似于语音识别和计算语言学中使用的概率建模方法被用于对酵母基因组进行计算筛选，并鉴定出22种甲基化指导snoRNAs，即snR50至snR71。基因破坏和其他实验表征证实了它们的甲基化指导功能。酵母核糖体RNA中55个核糖甲基化位点中的51个总共被分配给41种不同的指导snoRNAs。
