Victorov I, Nguyen-Legros J, Boutry J M, Gay M, Berger B, Hauw J J
C R Acad Hebd Seances Acad Sci D. 1978 Jun 26;286(25):1893-4.
The dissecting procedure and tissue culture technique on collagen coated coverslips in Leighton tubes, of newborn Mouse nucleus locus coeruleus and the regular histofluorescence demonstration of catecholaminergic fibers in the outgrowth zone are described. This simple method allows 10 day--long experiments .
本文描述了在新生小鼠蓝斑核上进行的解剖程序以及在莱顿管中胶原包被盖玻片上的组织培养技术,以及对生长区儿茶酚胺能纤维的常规组织荧光显示。这种简单的方法可以进行长达10天的实验。