Victorov I, Nguyen-Legros J, Boutry J M, Alvarez C, Hauw J J
Biomedicine. 1979 Jul;30(3):161-8.
A simple and reliable method of sampling explants from the nucleus of the Locus Coeruleus of newborn mice and their cultivation in Leighton tubes is described. This technique reliably provides cultures available for experiments lasting, at least, ten days. The cells of the nucleus of the Locus Coeruleus and newly formed norepinephrine-containing fibers are demonstrated by fluorescence histochemistry together with pharmacological tests. Their morphological characteristics before and after fixation and staining are described. Electron microscopy confirmed the healthy condition of these cultures.
本文描述了一种从新生小鼠蓝斑核中采集外植体并在莱顿管中培养的简单可靠方法。该技术能可靠地提供可用于至少持续十天实验的培养物。通过荧光组织化学结合药理学测试来展示蓝斑核细胞和新形成的含去甲肾上腺素纤维。描述了它们在固定和染色前后的形态特征。电子显微镜证实了这些培养物的健康状况。
