重组耶尔森菌外膜蛋白对由包膜和非包膜鼠疫耶尔森菌引起的腺鼠疫的保护效力。
Protective efficacy of recombinant Yersinia outer proteins against bubonic plague caused by encapsulated and nonencapsulated Yersinia pestis.
作者信息
Andrews G P, Strachan S T, Benner G E, Sample A K, Anderson G W, Adamovicz J J, Welkos S L, Pullen J K, Friedlander A M
机构信息
Division of Bacteriology, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA.
出版信息
Infect Immun. 1999 Mar;67(3):1533-7. doi: 10.1128/IAI.67.3.1533-1537.1999.
Abstract
To evaluate the role of Yersinia outer proteins (Yops) in conferring protective immunity against plague, six yop loci from Yersinia pestis were individually amplified by PCR, cloned, and expressed in Escherichia coli. The recombinant proteins were purified and injected into mice. Most Yop-vaccinated animals succumbed to infection with either wild-type encapsulated Y. pestis or a virulent, nonencapsulated isogenic variant. Vaccination with YpkA significantly prolonged mean survival time but did not increase overall survival of mice infected with the nonencapsulated strain. The only significant protection against death was observed in YopD-vaccinated mice challenged with the nonencapsulated strain.
摘要
为评估耶尔森氏菌外膜蛋白（Yops）在赋予鼠疫保护性免疫方面的作用，通过聚合酶链反应（PCR）分别扩增、克隆来自鼠疫耶尔森氏菌的6个yop基因座，并在大肠杆菌中表达。将重组蛋白纯化后注射到小鼠体内。大多数接种Yop的动物死于野生型包膜鼠疫耶尔森氏菌或无毒力、非包膜同基因变体的感染。接种YpkA可显著延长平均存活时间，但未提高感染非包膜菌株小鼠的总体存活率。在接种YopD的小鼠受到非包膜菌株攻击时，观察到了唯一显著的抗死亡保护作用。
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本文引用的文献
1
Immune response to Yersinia outer proteins and other Yersinia pestis antigens after experimental plague infection in mice.小鼠实验性鼠疫感染后对耶尔森氏菌外膜蛋白和其他鼠疫耶尔森氏菌抗原的免疫反应。
Infect Immun. 1999 Apr;67(4):1922-8. doi: 10.1128/IAI.67.4.1922-1928.1999.
2
Identification of p130Cas as a substrate of Yersinia YopH (Yop51), a bacterial protein tyrosine phosphatase that translocates into mammalian cells and targets focal adhesions.鉴定p130Cas为耶尔森氏菌YopH（Yop51）的底物，YopH是一种细菌蛋白酪氨酸磷酸酶，可转运至哺乳动物细胞并作用于粘着斑。
EMBO J. 1997 May 15;16(10):2730-44. doi: 10.1093/emboj/16.10.2730.
3
YopK of Yersinia pseudotuberculosis controls translocation of Yop effectors across the eukaryotic cell membrane.假结核耶尔森菌的YopK蛋白控制Yop效应蛋白穿过真核细胞膜的转运。
Mol Microbiol. 1997 Apr;24(1):73-91. doi: 10.1046/j.1365-2958.1997.3211681.x.
4
The Yersinia Yop virulon: a bacterial system for subverting eukaryotic cells.耶尔森氏菌Yop毒力蛋白分泌系统：一种用于颠覆真核细胞的细菌系统。
Mol Microbiol. 1997 Mar;23(5):861-7. doi: 10.1046/j.1365-2958.1997.2731623.x.
5
Effect of Yersinia pestis YopM on experimental plague.鼠疫耶尔森菌YopM对实验性鼠疫的影响。
Infect Immun. 1997 Mar;65(3):924-30. doi: 10.1128/IAI.65.3.924-930.1997.
6
7
8
Recombinant V antigen protects mice against pneumonic and bubonic plague caused by F1-capsule-positive and -negative strains of Yersinia pestis.重组V抗原可保护小鼠免受由鼠疫耶尔森氏菌F1荚膜阳性和阴性菌株引起的肺鼠疫和腺鼠疫。
Infect Immun. 1996 Nov;64(11):4580-5. doi: 10.1128/iai.64.11.4580-4585.1996.
9
Construction of defined F1 negative mutants of virulent Yersinia pestis.强毒力鼠疫耶尔森氏菌特定F1阴性突变体的构建
Contrib Microbiol Immunol. 1995;13:325-8.
10
Studies on the contribution of the F1 capsule-associated plasmid pFra to the virulence of Yersinia pestis.关于F1荚膜相关质粒pFra对鼠疫耶尔森菌毒力贡献的研究。
Contrib Microbiol Immunol. 1995;13:299-305.
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