Des Rosiers M H, Descarries L
C R Acad Hebd Seances Acad Sci D. 1978 Jul 17;287(3):153-6.
Vascular perfusion of all products required for primary fixation, postfixation, dehydration and embedding of nervous tissue in Epon permits radio-autographic detection of radioactivity accumulated in the central nervous system after intravenous injection of [3H]deoxyglucose. This histological technique should allow application of the deoxyglucose method at cellular if not subcellular level, since a high proportion of the tracer appears to be retained in situ in specimens adequately preserved for light and electron microscope radio-autography.
用环氧树脂对神经组织进行初次固定、后固定、脱水和包埋所需的所有产品进行血管灌注，能够在静脉注射[3H]脱氧葡萄糖后，对中枢神经系统中积累的放射性进行放射自显影检测。这种组织学技术应能在细胞水平（如果不是亚细胞水平的话）应用脱氧葡萄糖法，因为在为光镜和电镜放射自显影充分保存的标本中，很大一部分示踪剂似乎原位保留。
