Fansa H, Keilhoff G, Plogmeier K, Frerichs O, Wolf G, Schneider W
Department of Plastic, Reconstructive, and Hand Surgery, University of Magdeburg, Germany.
J Reconstr Microsurg. 1999 Jan;15(1):61-5. doi: 10.1055/s-2007-1000072.
Acellular muscle grafts can support axonal regeneration over short gaps. Due to the lack of viable Schwann cells in the grafts, failure of regeneration is evident with increasing gap lengths. To create a biological nerve conduit, Schwann cells were implanted into acellular muscle. The grafts were then incubated in vitro and assessed histologically and morphometrically. For cultivation of the Schwann cells, rat sciatic nerves were allowed to predegenerate to obtain a high cell yield. Rat gracilis muscles were harvested and made acellular by a liquid nitrogen treatment. After Schwann cell implantation, the muscles were incubated in vitro for 2, 5, and 7 days. S100-immunostaining, NGF, and N-cadherin, characterized the Schwann cells within the muscle. Viability was assessed by fluoresceine-fluorescence staining. Proliferation was determined by BrdU-DNA incorporation. Cell implantation did not to affect Schwann cell viability. Cells were seen throughout the entire length of the muscle basal lamina. They aligned and formed a cell column. Immunostained for S-100, implanted cells showed 100 percent staining. N-cadherin and NGF were expressed by all of the S-100 positive cells. Predegeneration is considered to be a highly efficacious method, if a high yield of activated Schwann cells is required. The successful implantation of the cells into an acellular muscle provides the possibility of a biologic conduit, offering the advantage of large basal lamina tubes serving as a pathway for regenerating axons. It also provides the beneficial effects of viable Schwann cells that produce neurotrophic and neurotropic factors to support axonal regeneration. Functional outcomes require evaluation in further in vivo studies.
去细胞肌肉移植物可支持短间隙的轴突再生。由于移植物中缺乏活的施万细胞，随着间隙长度增加，再生失败很明显。为了创建生物神经导管，将施万细胞植入去细胞肌肉中。然后将移植物在体外培养，并进行组织学和形态学评估。为了培养施万细胞，使大鼠坐骨神经预先变性以获得高产率的细胞。收获大鼠股薄肌并通过液氮处理使其去细胞化。施万细胞植入后，将肌肉在体外培养2、5和7天。S100免疫染色、神经生长因子（NGF）和N-钙黏着蛋白可表征肌肉内的施万细胞。通过荧光素荧光染色评估细胞活力。通过溴脱氧尿苷（BrdU）掺入法测定细胞增殖。细胞植入不影响施万细胞活力。在肌肉基膜的整个长度上都可见到细胞。它们排列并形成细胞柱。植入的细胞经S-100免疫染色显示100%染色。所有S-100阳性细胞均表达N-钙黏着蛋白和NGF。如果需要高产率的活化施万细胞，预先变性被认为是一种高效的方法。将细胞成功植入去细胞肌肉中提供了形成生物导管的可能性，具有大的基膜管作为再生轴突通路的优势。它还提供了活的施万细胞产生神经营养因子和向神经因子以支持轴突再生的有益作用。功能结果需要在进一步的体内研究中进行评估。
