Gerkema M P, Shinohara K, Kimura F
Physiology Department, Yokohama City University Medical School, Yokohama, Netherlands.
Neurosci Lett. 1999 Jan 8;259(2):107-10. doi: 10.1016/s0304-3940(98)00919-7.
Organotypic hypothalamic cultures of neonatal rats comprising the suprachiasmatic nuclei (SCN) produce stable 20 h release patterns of vasoactive intestinal polypeptide (VIP) and arginine-vasopressin (AVP). Compared with rats, voles show variably expressed circadian activity patterns. In this study we measured neuropeptidergic release patterns in organotypic SCN cultures of neonatal common voles (Microtus arvalis, n = 6). Slices were prepared at postnatal day 6. After 14 days of incubation, 2 h samples of medium were collected during 50 h. None of the vole SCN slices showed a circadian modulation in VIP release. Peaks in AVP occurred, 20 h apart from each other, in four of six vole SCN slices. These findings contrast with the concurrent release patterns of VIP and AVP in rat SCN slices. The results suggest an independent role of both neuropeptides in the oscillatory output pathways of the circadian pacemaker in the common vole.
包含视交叉上核（SCN）的新生大鼠下丘脑器官型培养物可产生稳定的血管活性肠肽（VIP）和精氨酸加压素（AVP）20小时释放模式。与大鼠相比，田鼠表现出变化多样的昼夜活动模式。在本研究中，我们测量了新生普通田鼠（Microtus arvalis，n = 6）下丘脑器官型SCN培养物中的神经肽释放模式。在出生后第6天制备切片。孵育14天后，在50小时内每2小时收集一次培养基样本。没有一只田鼠的SCN切片显示VIP释放有昼夜节律调节。在六只田鼠的SCN切片中有四只，AVP的峰值彼此间隔20小时出现。这些发现与大鼠SCN切片中VIP和AVP的同时释放模式形成对比。结果表明这两种神经肽在普通田鼠昼夜节律起搏器的振荡输出途径中具有独立作用。
