Shinohara K, Honma S, Katsuno Y, Abe H, Honma K
Department of Physiology, Hokkaido University School of Medicine, Sapporo, Japan.
Neurosci Lett. 1994 Mar 28;170(1):183-6. doi: 10.1016/0304-3940(94)90269-0.
Temporal profiles of the amount of vasoactive intestinal polypeptide (VIP) were examined in the medium of organotypic suprachiasmatic nucleus (SCN) slice cultures over a 2-day period. Arginine-vasopressin (AVP) level was also measured in the same medium. The slices of the SCN were obtained from 7-8-day-old rats and cultured individually in tubes on a roller drum for 14 days. The VIP amount in the medium of SCN culture showed a circadian rhythm with a approximately 22-h period. Circadian rhythms with identical periods were also observed in AVP amount of the same culture. However, the peak time of the VIP rhythm was slightly ahead of that of the AVP rhythm. Furthermore, the total VIP amount in the medium over a 24-h period was six times as large as that of AVP. These results suggest that there is a circadian rhythm of VIP which is released from the ventrolateral SCN.
在为期2天的时间里，对器官型视交叉上核（SCN）切片培养物的培养基中血管活性肠肽（VIP）的含量进行了时间分布研究。同时也测量了同一培养基中精氨酸加压素（AVP）的水平。SCN切片取自7 - 8日龄大鼠，并在滚筒上的试管中单独培养14天。SCN培养物培养基中的VIP含量呈现出约22小时周期的昼夜节律。在同一培养物的AVP含量中也观察到了相同周期的昼夜节律。然而，VIP节律的峰值时间略早于AVP节律的峰值时间。此外，24小时内培养基中VIP的总量是AVP总量的6倍。这些结果表明，存在一种从腹外侧SCN释放的VIP昼夜节律。
