Böcking A, Grossarth C, Deimling O V
Histochemistry. 1976 Nov 19;50(1):65-76. doi: 10.1007/BF00492787.
The intracellular distribution of non specific esterases in various villous cells of mouse jejunum was investigated using two substrates, 8-acetoxiquinoline (Q-O-2) and 8-acetyl mercaptoquinoline (Q-S-2) respectively. With the more selectively staining Q-S-2 a uniform reaction was demonstrated in all enterocytes which was mainly located in the endoplasmic reticulum and the nuclear envelope. Possibly it is a matter of a single enzyme being hardly detachable from the membranes. With the non selectively staining Q-O-2 several esterases were demonstrated Es-2, adn Es-9 among them. The main reaction was found in columnar and Goblet cells, in both at all cellular membranes and in the mitochondrial matrix, additionally in cisterns and vesicles of the endoplasmic reticulum and of the Golgi apparatus and at lipid droplets of the former, finally at mucous droplets of the latter. An extracellular reaction was found in the intercellular cleft and between the microvilli of the brush border.
分别使用两种底物8-乙酰氧基喹啉（Q-O-2）和8-乙酰巯基喹啉（Q-S-2）研究了小鼠空肠不同绒毛细胞中非特异性酯酶的细胞内分布。使用选择性更强的Q-S-2染色时，所有肠细胞均呈现均匀反应，主要位于内质网和核膜。这可能是由于一种单一酶很难从膜上分离下来。使用非选择性染色的Q-O-2时，显示出几种酯酶，其中包括Es-2和Es-9。主要反应见于柱状细胞和杯状细胞，在所有细胞膜和线粒体基质中均有，此外在内质网和高尔基体的池和小泡中以及前者的脂滴处有反应，最后在后者的粘液滴处有反应。在细胞间裂隙和刷状缘微绒毛之间发现了细胞外反应。
