Böcking A, Riede U N
J Histochem Cytochem. 1979 May;27(5):967-74. doi: 10.1177/27.5.479558.
Changes in ultrastructural distribution patterns of nonspecific esterases (E.C. 3.1.19) are described quantitatively by means of morphometry. Esterases were demonstrated with O-acetyl-8-hydroxyquinoline (Q-O-2) and S-acetyl-8-mercaptoquinoline in livers of normally and exclusively fructose-fed mice. Conditions are discussed, under which the quantification of the ultrastructural products of enzyme histochemical reactions may be possible. Smooth endoplasmic reticulum and rough endoplasmic reticulum exhibit no alteration in enzyme distribution with both substrates since the enzyme-occupied proportion of each compartment remains the same despite an overall decrease of both compartments. Likewise an increase of O-acetyl-8-hydroxyquinoline-esterase at fat droplets corresponds to the increase in total surface of the fat. S-acetyl-8-mercaptoquinoline-positive fat surface however reveals an increase far beyond that of the total fat surface. The results support the hypothesis that a variety of esterases with different substrate spectra are present at the subcellular level in different cell compartments.
运用形态计量学方法定量描述了非特异性酯酶（E.C. 3.1.19）超微结构分布模式的变化。在正常饮食和仅喂食果糖的小鼠肝脏中，使用O - 乙酰 - 8 - 羟基喹啉（Q - O - 2）和S - 乙酰 - 8 - 巯基喹啉对酯酶进行了显示。文中讨论了对酶组织化学反应的超微结构产物进行定量的可能条件。滑面内质网和粗面内质网在两种底物作用下酶分布均无变化，因为尽管每个区室总体减少，但每个区室被酶占据的比例保持不变。同样，脂肪滴处O - 乙酰 - 8 - 羟基喹啉酯酶的增加与脂肪总表面积的增加相对应。然而，S - 乙酰 - 8 - 巯基喹啉阳性脂肪表面的增加远远超过总脂肪表面的增加。这些结果支持了这样一种假说，即在亚细胞水平的不同细胞区室中存在具有不同底物谱的多种酯酶。
