Municio del Campo M J, Villameytide M L
Laboratorio de Bioquímica, Fundación Jiménez Díaz, Madrid, Spain.
Cancer Lett. 1998 Dec 25;134(2):147-53. doi: 10.1016/s0304-3835(98)00245-6.
The aim of this work was to study the regulation by calcium of the synthesis of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) in Walker 256 carcinosarcoma (W256) cells compared to renal phenotype LLC-PK1 cells. We have detected synthesis of 1,25(OH)2D3 and 24,25(OH)2D3 in W256 cells. Levels of 1,25(OH)2D3 in W256 were similar to those of LLC-PK1 cells. Levels of 24,25(OH)2D3 were higher in W256 than in LLC-PK1 cells. High levels of calcium inhibited 1,25(OH)2D3 synthesis in LLC-PK1 cells but not in W256 cells.
本研究的目的是，与肾表型LLC-PK1细胞相比，研究钙对Walker 256癌肉瘤（W256）细胞中1,25-二羟基维生素D3（1,25(OH)2D3）和24,25-二羟基维生素D3（24,25(OH)2D3）合成的调节作用。我们已经检测到W256细胞中1,25(OH)2D3和24,25(OH)2D3的合成。W256细胞中1,25(OH)2D3的水平与LLC-PK1细胞相似。W256细胞中24,25(OH)2D3的水平高于LLC-PK1细胞。高浓度的钙抑制LLC-PK1细胞中1,25(OH)2D3的合成，但不抑制W256细胞中1,25(OH)2D3的合成。
