Effects of 24R,25-dihydroxyvitamin D3 on alkaline phosphatase activity in pig renal epithelial LLC-PK1 cells in culture.
作者信息
Thanh X D, Massicot F, Do B, Breget R, Durand D, Warnet J M, Claude J R, Taniguchi N, Clot J P
机构信息
Laboratoire de Pharmacologie/CHU-UFR de Pharmacie, Université d'Angers, France.
出版信息
Gen Pharmacol. 1998 Sep;31(3):459-62. doi: 10.1016/s0306-3623(98)00011-1.
PMID:9703220
Abstract
- The effects of 24R,25-dihydroxyvitamin D3 [24,25(OH)2D3] on alkaline phosphatase activity (ALP) were evaluated in pig kidney LLC-PK1 cells in culture. 2. The vitamin D3 metabolite increased ALP activity in these cells, whereas no effect of the hormone was observed on gamma-glutamyltranspeptidase and acid phosphatase activities. 3. ALP activity was stimulated after 3- to 12-hr incubation in the presence of 10(-9) mol/l 24,25(OH)2D3 with a maximum after 6 hr. 4. The hormonal induction of ALP activity was prevented by pretreatment of cells by actinomycin D. 5. It is proposed that 24,25(OH)2D3 could increase ALP activity by de novo protein synthesis.
摘要
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