Shin E C, Shin J S, Park J H, Kim J J, Kim H, Kim S J
Department of Microbiology, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, South Korea.
Cancer Lett. 1998 Dec 25;134(2):155-62. doi: 10.1016/s0304-3835(98)00251-1.
Hepatocellular carcinoma (HCC) is one of the tumors known to be resistant to Fas-mediated apoptosis. To elucidate the possible mechanisms of this resistance, we examined the mRNA transcripts of genes related to Fas in 10 hepatitis B virus (HBV)-associated HCC cell lines and six HBV-associated HCC tissues. Most of the HBV-associated HCC cell lines showed markedly decreased Fas expression. Some cell lines expressed FAP, a possible inhibitor of apoptosis, or showed downregulated expression of FADD or FLICE, molecules essential to Fas-mediated apoptosis. All six HBV-associated HCC tissues also showed decreased Fas expression compared with their non-malignant counterparts. Our data demonstrate that HBV-associated HCC showed a common defect in the expression of Fas, upregulation of FAP and downregulation of downstream molecules such as FADD and FLICE.
肝细胞癌（HCC）是已知对Fas介导的凋亡具有抗性的肿瘤之一。为了阐明这种抗性的可能机制，我们检测了10种乙型肝炎病毒（HBV）相关的HCC细胞系和6个HBV相关的HCC组织中与Fas相关基因的mRNA转录本。大多数HBV相关的HCC细胞系显示Fas表达明显降低。一些细胞系表达FAP（一种可能的凋亡抑制剂），或显示FADD或FLICE（Fas介导凋亡所必需的分子）表达下调。与非恶性对应组织相比，所有6个HBV相关的HCC组织也显示Fas表达降低。我们的数据表明，HBV相关的HCC在Fas表达、FAP上调以及FADD和FLICE等下游分子下调方面存在共同缺陷。
