Wakabayashi H, Natsuka S, Mega T, Otsuki N, Isaji M, Naotsuka M, Koyama S, Kanamori T, Sakai K, Hase S
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
J Biol Chem. 1999 Feb 26;274(9):5436-42. doi: 10.1074/jbc.274.9.5436.
O-linked sugar chains with xylose as a reducing end linked to human urinary soluble thrombomodulin were studied. Sugar chains were liberated by hydrazinolysis followed by N-acetylation and tagged with 2-aminopyridine. Two fractions containing pyridylaminated Xyl as a reducing end were collected. Their structures were determined by partial acid hydrolysis, two-dimensional sugar mapping combined with exoglycosidase digestions, methylation analysis, mass spectrometry, and NMR as SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1+ ++-4Xyl. These sugar chains could bind to an HNK-1 monoclonal antibody. This is believed to be the first example of a proteoglycan linkage tetrasaccharide with glucuronic acid 3-sulfate and sialic acid.
对以木糖为还原端与人类尿液可溶性血栓调节蛋白相连的O-连接糖链进行了研究。糖链经肼解、N-乙酰化后释放出来，并用2-氨基吡啶进行标记。收集到两个以还原端含吡啶氨基化木糖的级分。通过部分酸水解、二维糖图谱结合外切糖苷酶消化、甲基化分析、质谱和核磁共振确定其结构为SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1+ ++-4Xyl。这些糖链可与HNK-1单克隆抗体结合。据信这是具有3-硫酸化葡萄糖醛酸和唾液酸的蛋白聚糖连接四糖的首个实例。
