Wakabayashi H, Natsuka S, Honda M, Naotsuka M, Ito Y, Kajihara J, Hase S
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
J Biochem. 2001 Oct;130(4):543-52. doi: 10.1093/oxfordjournals.jbchem.a003017.
The sugar chains of human urinary thrombomodulin were studied. N- and O-linked sugar chains were simultaneously liberated by hydrazinolysis followed by N-acetylation and were tagged with 2-aminopyridine. Then the structures of the N- and O-linked pyridylamino (PA-) sugar chains were analyzed by two-dimensional sugar mapping combined with exoglycosidase digestion. The major N-linked sugar chains of human urinary thrombomodulin were found to be monosialo- and disialofucosylbiantennary chains, while the major O-linked sugar chain was +/-Siaalpha2-3Galbeta1-3(+/-Siaalpha2-6)GalNAc. Thrombomodulin also contained the reported structure SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1-4Xyl [H. Wakabayashi, S. Natsuka, T. Mega, N. Otsuki, M. Isaji, M. Naotsuka, S. Koyama, T. Kanamori, K. Sakai, and S. Hase (1999) J. Biol. Chem. 274, 5436-5442]. In addition to these sugar chains, a single Glc was linked to Ser 287.
对人尿血栓调节蛋白的糖链进行了研究。通过肼解同时释放N-连接和O-连接的糖链，随后进行N-乙酰化，并用2-氨基吡啶进行标记。然后，通过二维糖图谱结合外切糖苷酶消化分析N-连接和O-连接的吡啶氨基（PA-）糖链的结构。发现人尿血栓调节蛋白的主要N-连接糖链是单唾液酸和双唾液酸岩藻糖基二天线链，而主要的O-连接糖链是+/-Siaα2-3Galβ1-3（+/-Siaα2-6）GalNAc。血栓调节蛋白还含有报道的结构SO4-3GlcAβ1-3Galβ1-3（+/-Siaα2-6）Galβ1-4Xyl [H. Wakabayashi, S. Natsuka, T. Mega, N. Otsuki, M. Isaji, M. Naotsuka, S. Koyama, T. Kanamori, K. Sakai, and S. Hase (1999) J. Biol. Chem. 274, 5436-5442]。除了这些糖链外，一个单一的Glc与Ser 287相连。
