Nishikawa S, Sasaki F
Department of Biology, Tsurumi University School of Dental Medicine, Yokohama, Japan.
J Histochem Cytochem. 1999 Mar;47(3):303-12. doi: 10.1177/002215549904700304.
Apoptosis of dental pulp cells of rat incisors was investigated by the TUNEL method and electron microscopy. The results showed that a considerable amount of apoptosis occurred in the pulp, increasing in extent with incisal direction. OX6, ED1, and ED2 antibodies were used to detect macrophages and dendritic cells in combination with immunoelectron microscopy. Apoptotic fragments were eliminated mainly by MHC Class II-expressing cells, including dendritic cells positive for the OX6 antibody, and by MHC Class II-negative macrophages. Macrophages and dendritic cells positive for OX6, ED1, or ED2 increased from the apical to incisal direction of the incisor. These results indicate that apoptosis contributes to normal pulp formation and maintenance.
采用TUNEL法和电子显微镜研究大鼠切牙牙髓细胞的凋亡。结果显示，牙髓中发生了大量凋亡，且凋亡程度沿切端方向增加。使用OX6、ED1和ED2抗体结合免疫电子显微镜检测巨噬细胞和树突状细胞。凋亡碎片主要由表达MHC II类分子的细胞清除，包括OX6抗体阳性的树突状细胞以及MHC II类分子阴性的巨噬细胞。OX6、ED1或ED2阳性的巨噬细胞和树突状细胞从切牙的根尖向切端方向增多。这些结果表明，凋亡有助于牙髓的正常形成和维持。
