一种来自牛胆囊胆汁的新型胆汁酸结合物——纤毛胆酸。

A new bile acid conjugate, ciliatocholic acid, from bovine gall bladder bile.

作者信息

Tamari M, Ogawa M, Kametaka M

出版信息

J Biochem. 1976 Aug;80(2):371-7. doi: 10.1093/oxfordjournals.jbchem.a131286.

DOI:10.1093/oxfordjournals.jbchem.a131286

Abstract

This study was carried out to investigate the occurrence of ciliatocholic acid in bovine gall bladder bile. Ciliatocholic acid was synthesized according to the method described by Bergstrôm and Norman for the synthesis of taurocholic acid. Elemental analysis, melting point, and the infrared spectrum of this substance were determined. An isolation procedure for ciliatocholic acid was established by stepwise elution with an HCl-ethanol solvent system using a Dowex-1 anion exchange resin column chromatographic technique. Ciliatocholic acid amounting to 158 mug (as ciliatine) per 100 ml of gall bladder bile was found in the fraction eluted with 0.01 N HCl in 50% ethanol. This coumpound was purified by preparative thin-layer chromatography and confirmed to be ciliatocholic acid from the hydrolytic stability, phosphorus determination, and chromatographic behavior. Thus, bovine gall bladder bile contains a small amount of ciliatocholic acid.

摘要

本研究旨在调查牛胆囊胆汁中毛束胆汁酸的存在情况。毛束胆汁酸是根据Bergstrôm和Norman描述的牛磺胆酸合成方法合成的。测定了该物质的元素分析、熔点和红外光谱。采用Dowex - 1阴离子交换树脂柱色谱技术，通过用HCl - 乙醇溶剂系统逐步洗脱，建立了毛束胆汁酸的分离方法。在用50%乙醇中的0.01 N HCl洗脱的馏分中，发现每100 ml胆囊胆汁中含有158 μg（以毛束碱计）的毛束胆汁酸。该化合物通过制备薄层色谱法纯化，并通过水解稳定性、磷测定和色谱行为确认为毛束胆汁酸。因此，牛胆囊胆汁中含有少量毛束胆汁酸。