Tsutsumi K, Majima R, Shimura K
J Biochem. 1976 Nov;80(5):1039-45. doi: 10.1093/oxfordjournals.jbchem.a131359.
The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis. Analysis of the incorporation of the methyl group from [methyl-14C]methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4.5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step.
通过脉冲追踪标记和DNA-RNA杂交竞争实验，证明了家蚕后部丝腺多核糖体后组分中存在tRNA前体。这些前体在聚丙烯酰胺凝胶电泳上的分子大小范围为4S至5S。对[甲基-14C]甲硫氨酸中甲基掺入的分析表明，在短暂标记期间，聚丙烯酰胺凝胶上的一个放射性峰出现在4.5S区域。这表明在4.5S前体步骤发生了一些甲基化。
