转运RNA的生物合成：大肠杆菌酶将家蚕转运RNA前体体外转化为4S RNA
Biosynthesis of transfer RNA: in vitro conversion of transfer RNA precursors from Bombyx mori to 4S RNA by Escherichia coli enzymes.
作者信息
Chen G S, Siddiqui M A
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2610-3. doi: 10.1073/pnas.70.9.2610.
Abstract
Evidence for the presence of rapidly labeled short-lived RNA species, presumed to be precursors to transfer RNA (tRNA), in the silk glands of Bombyx mori is presented. These precursors, which migrate between 4S and 5S markers on acrylamide gels, can be converted in vitro into molecules indistinguishable in size from tRNA. The conversion is also catalyzed by a crude enzyme fraction isolated from ribosomes of E. coli. Since a similar enzyme preparation cleaves the suppressor tRNA(Tyr) sequence of E. coli from its precursor molecule, it would appear that the precursor to E. coli tRNA(Tyr) and the precursors to silk-gland tRNAs share common structural features.
摘要
本文提供了家蚕丝腺中存在快速标记的短寿命RNA种类（推测为转运RNA（tRNA）前体）的证据。这些前体在丙烯酰胺凝胶上于4S和5S标记之间迁移，可在体外转化为大小与tRNA无法区分的分子。这种转化也由从大肠杆菌核糖体分离的粗酶组分催化。由于类似的酶制剂能从其前体分子上切割大肠杆菌的抑制性tRNA(Tyr)序列，因此看来大肠杆菌tRNA(Tyr)的前体和丝腺tRNA的前体具有共同的结构特征。
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Biosynthesis of transfer RNA: in vitro conversion of transfer RNA precursors from Bombyx mori to 4S RNA by Escherichia coli enzymes.转运RNA的生物合成：大肠杆菌酶将家蚕转运RNA前体体外转化为4S RNA
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本文引用的文献
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Functional adaptation of tRNAs to fibroin biosynthesis in the silkgland of Bombyx mori L.家蚕（Bombyx mori L.）丝腺中转运RNA（tRNA）对丝心蛋白生物合成的功能适应性
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Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分离多种核糖核酸种类
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Enzymic conversion of tRNA precursor to 4-S RNA in vitro.体外将tRNA前体酶促转化为4-S RNA
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Transfer RNA synthesis in HeLa cells. II. Formation of tRNA from a precursor in vitro and formation of pseudouridine.海拉细胞中的转运RNA合成。II. 体外由前体形成tRNA及假尿苷的形成
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Intracellular location and molecular characteristics of tumour cell transfer RNA precursors.肿瘤细胞转运RNA前体的细胞内定位及分子特征
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In vitro synthesis of tRNA Tyr precursors and their conversion to 4S RNA.酪氨酸转运核糖核酸前体的体外合成及其向4S核糖核酸的转化。
Nat New Biol. 1971 Sep 15;234(50):198-201. doi: 10.1038/newbio234198a0.
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Isolation of tyrosine tRNA precursor molecules.酪氨酸tRNA前体分子的分离
Nat New Biol. 1971 Jan 6;229(1):19-21. doi: 10.1038/newbio229019a0.
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Nat New Biol. 1971 Sep 8;233(36):35-9. doi: 10.1038/newbio233035a0.
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