Quernin M H, Poonkuzhali B, Médard Y, Dennison D, Srivastava A, Krishnamoorthy R, Chandy M, Jacqz-Aigrain E
Pediatric Clinical Pharmacology, Unité de Pharmacologie Clinique Pédiatrique Hôpital Robert Debré, Paris, France.
J Chromatogr B Biomed Sci Appl. 1999 Jan 8;721(1):147-52. doi: 10.1016/s0378-4347(98)00473-3.
A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement.
建立了一种高效液相色谱（HPLC）法并验证其用于测定血浆中白消安的含量。白消安用甲苯萃取，经2,3,5,6-四氟硫酚衍生化得到二-TFTP-丁烷，然后将衍生化产物再用甲苯萃取并注入具有紫外检测（波长：275nm）的HPLC系统。萃取回收率为80%，定量限为50ng/ml，线性范围为50至2000ng/ml。此外，基于相同的衍生化程序，用HPLC和GC-MS分析法对42份接受白消安治疗的儿科患者的样本进行了分析。二-TFTP-丁烷浓度之间的相关性非常显著（p<0.0001），表明这两种方法具有良好的一致性。
