Peris J E, Latorre J A, Castel V, Verdeguer A, Esteve S, Torres-Molina F
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Valencia, Spain.
J Chromatogr B Biomed Sci Appl. 1999 Jun 25;730(1):33-40. doi: 10.1016/s0378-4347(99)00214-5.
A rapid, sensitive and reproducible high-performance liquid chromatographic assay for busulfan in human plasma was developed. After extraction of plasma samples with acetonitrile and methylene chloride, busulfan and the internal standard [1,5-bis(methanesulfonyloxy)pentane] were derivatized with 8-mercaptoquinoline to yield fluorescent compounds which were detected with a fluorescence detector equipped with filters of 360 nm (excitation) and 425 nm (emission). Calibration graphs showed a linear correlation (r>0.9990) over the concentration range of 20-2000 ng/ml. The recovery of busulfan from plasma standards was 70+/-5%. The detection and quantification limits for busulfan in plasma samples were established at 9 ng/ml and 20 ng/ml, respectively. The intra- and inter-assay variations were lower than 8% and 10%, respectively. The applicability of the method was verified by analyzing the plasma concentrations of busulfan in a patient to whom it was administered orally on two different days.
建立了一种快速、灵敏且可重复的人血浆中白消安的高效液相色谱测定法。用乙腈和二氯甲烷萃取血浆样品后，白消安和内标物[1,5-双（甲磺酰氧基）戊烷]用8-巯基喹啉衍生化，生成荧光化合物，用配备360nm（激发）和425nm（发射）滤光片的荧光检测器进行检测。校准曲线在20-2000ng/ml的浓度范围内呈线性相关（r>0.9990）。血浆标准品中白消安的回收率为70±5%。血浆样品中白消安的检测限和定量限分别确定为9ng/ml和20ng/ml。批内和批间变异分别低于8%和10%。通过分析在两天不同时间口服白消安的患者的血浆浓度，验证了该方法的适用性。
