Blandini F, Conti G, Martignoni E, Colangelo V, Nappi G, Di Grezia R, Orzi F
Neurological Institute C Mondino, University of Pavia, Italy.
J Cereb Blood Flow Metab. 1999 Feb;19(2):149-54. doi: 10.1097/00004647-199902000-00006.
Inactivation of the subthalamic nucleus (STN) has attracted interest as a therapeutic tool in Parkinson's disease. The functional consequences of the inactivation, however, are uncertain. In this study definition of the pattern of changes of cerebral functional activity associated with lesion of the STN and dopaminergic stimulation, by using the [14C]deoxyglucose method, was sought. Six or 7 days following unilateral lesion of the STN, the animals were divided into two groups: One group (n = 10) was administered apomorphine (1 mg/kg) subcutaneously; the second group (n = 10) received saline. The [14C]deoxyglucose procedure was initiated 10 minutes following the drug or saline injection. The results show that systemic administration of apomorphine to rats with unilateral lesion of the STN causes ipsiversive rotational behavior and asymmetries of glucose utilization of defined brain areas, including the substantia nigra reticulata, globus pallidus, and entopeduncular nucleus. These nuclei are the main targets of the subthalamic excitatory projections. Lesion of the nucleus per se (without challenge with apomorphine) has no significant consequences on glucose utilization. The findings indicate that the STN is involved in the activation of the basal ganglia output nuclei induced by systemic dopaminergic stimulation.
丘脑底核（STN）失活作为帕金森病的一种治疗手段已引起关注。然而，失活的功能后果尚不确定。在本研究中，试图通过使用[14C]脱氧葡萄糖法来明确与STN损伤及多巴胺能刺激相关的脑功能活动变化模式。在STN单侧损伤6或7天后，将动物分为两组：一组（n = 10）皮下注射阿扑吗啡（1 mg/kg）；第二组（n = 10）注射生理盐水。在注射药物或生理盐水10分钟后开始[14C]脱氧葡萄糖程序。结果显示，对单侧STN损伤的大鼠全身给予阿扑吗啡会导致向损伤侧旋转行为以及特定脑区葡萄糖利用的不对称，这些脑区包括黑质网状部、苍白球和内苍白球核。这些核团是丘脑底核兴奋性投射的主要靶点。核团本身的损伤（未用阿扑吗啡激发）对葡萄糖利用无显著影响。研究结果表明，STN参与了全身多巴胺能刺激诱导的基底神经节输出核团的激活。
