A comparison of the determination of partition coeficients of 1,4-benzodiazephines by high-performance liquid chromatography and thin-layer chromatography.

Relative partition coefficients (P) of 1,4-benzodiazepines were determined by a reversed-phase thin-layer chromatographic (RP-TLC) technique in the systemoleyl alcohol-water, and by high-performance liquid chromatography (HPLC) using several column packing materials. RMw values, obtained by RP-TLC, correlated well with log P values determined directly in the system oleyl alcohol-water and with the literature values for the system 1-octanol-water. Th log k' w values from the HPLC experiments could be determined with greater precision, but the correlations of log k'w with log Poleyl alcohol and log Poctanol were not as good as those found for the Rmw values.