高效液相色谱法在血浆中某些1,4-苯二氮䓬类及其代谢物测定中的应用。
The application of HPLC to the determination of some 1,4 benzodiazepines and their metabolites in plasma.
作者信息
Skellern G G, Meier J, Knight B I, Whiting B
出版信息
Br J Clin Pharmacol. 1978 Jun;5(6):483-7. doi: 10.1111/j.1365-2125.1978.tb01661.x.
Abstract
1 A high pressure liquid chromatographic (HPLC) assay has been developed for the measurement of chlordiazepoxide and its metabolites, N-desmethylchlordiazepoxide and demoxepam in microgram concentrations in human plasma. 2 Another metabolite was detected in appreciable amounts in the plasma from patients receiving high doses of chlordiazepoxide after the first 3 days of treatment. 3 Preliminary studies would indicate that this metabolite is N-desmethyldiazepam. 4 A modified HPLC system, using a microparticulate column was developed to separate N-desmethyldiazepam from chlordiazepoxide and its other metabolites in plasma.
摘要
- 已开发出一种高压液相色谱（HPLC）分析法，用于测定人血浆中微克浓度的氯氮卓及其代谢产物N-去甲基氯氮卓和去氧安定。2. 在接受高剂量氯氮卓治疗3天后的患者血浆中，还检测到了另一种含量可观的代谢产物。3. 初步研究表明，这种代谢产物是N-去甲基地西泮。4. 开发了一种使用微粒柱的改良HPLC系统，以从血浆中的氯氮卓及其其他代谢产物中分离出N-去甲基地西泮。
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Anal Chem. 1973 Sep;45(11):1846-51. doi: 10.1021/ac60333a040.
3
The determination of some 1,4-benzodiazepines and their metabolites in body fluids. A review.
Analyst. 1974 May;99(1178):241-72. doi: 10.1039/an9749900241.
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Chromatography of the 1,4-benzodiazepines.
J Chromatogr. 1974 Oct 30;98(3):527-68. doi: 10.1016/s0021-9673(00)84071-6.
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