Dyer R D, Sarto G E, Colás A E
J Clin Endocrinol Metab. 1976 Dec;43(6):1211-8. doi: 10.1210/jcem-43-6-1211.
Progesterone binding by 100,000 X g cytosols of human myometria from patients undergoing hysterectomy for various reasons was assayed using equilibrium dialysis or DEAE-cellulose filtration. The estimates of high-affinity porgesterone binding obtained by both techniques showed a significant correlation. Dialysis and filtration assays with cortisol competing with [3H]progesterone for binding indicated that the specific progesterone binding by the cytosols was the result of progesterone interaction with two high-affinity constitutents. Myometrial cytosols from tissues under progestin stimulation (secretory endometrium or pregnancy) had low concentrations of progestin binders. Cytosols from uteri with atrophic endometrium showed consistently high concentrations of progestin binders and those tissues under estrogen stimulation (proliferative endometrium or hyperplasia) and variable binder concentrations. No differences were detected between grouped observtions classified into 10 clinical categories. The DEAE-cellulose filtration is an effective method for evaluating progesterone binding which uses well-established statistical techniques and requires less tissue, less time for incubations, and fewer points for reliable analysis than does equilibrium dialysis.
采用平衡透析法或二乙氨基乙基纤维素（DEAE-纤维素）过滤法，对因各种原因接受子宫切除术患者的人子宫肌层100,000×g胞质溶胶中的孕酮结合情况进行了测定。通过这两种技术获得的高亲和力孕酮结合估计值显示出显著相关性。用皮质醇与[3H]孕酮竞争结合的透析和过滤试验表明，胞质溶胶中特异性孕酮结合是孕酮与两种高亲和力成分相互作用的结果。来自孕激素刺激组织（分泌期子宫内膜或妊娠）的子宫肌层胞质溶胶中孕激素结合蛋白浓度较低。来自萎缩性子宫内膜子宫的胞质溶胶中孕激素结合蛋白浓度始终较高，而来自雌激素刺激组织（增殖期子宫内膜或增生）的子宫的胞质溶胶中结合蛋白浓度则各不相同。在分为10个临床类别的分组观察中未检测到差异。DEAE-纤维素过滤法是一种评估孕酮结合的有效方法，该方法采用成熟的统计技术，与平衡透析法相比，所需组织更少、孵育时间更短、可靠分析所需的点数更少。
