Coenye T, Holmes B, Kersters K, Govan J R, Vandamme P
Laboratory of Microbiology, Universiteit Gent, Belgium.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:37-42. doi: 10.1099/00207713-49-1-37.
Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.
使用全细胞蛋白的SDS-PAGE、DNA-DNA杂交和广泛的生化特性分析,将椰毒伯克霍尔德菌和万氏伯克霍尔德菌的参考菌株与其他伯克霍尔德菌属的菌株进行了比较。唐菖蒲伯克霍尔德菌和椰毒伯克霍尔德菌在化学分类和生化分析中无法区分。类鼻疽伯克霍尔德菌和万氏伯克霍尔德菌具有无法区分的全细胞蛋白图谱,但万氏伯克霍尔德菌模式菌株在几项生化试验中与类鼻疽伯克霍尔德菌菌株不同。DNA-DNA结合水平(高于70%)表明:(i)唐菖蒲伯克霍尔德菌和椰毒伯克霍尔德菌,以及(ii)类鼻疽伯克霍尔德菌和万氏伯克霍尔德菌各自代表一个单一物种。得出结论:椰毒伯克霍尔德菌和万氏伯克霍尔德菌分别是唐菖蒲伯克霍尔德菌和类鼻疽伯克霍尔德菌的次异名。