Department of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu, 632004, India.
Ann Clin Microbiol Antimicrob. 2019 Feb 4;18(1):7. doi: 10.1186/s12941-019-0306-0.
Bacteria belonging to the Burkholderia cepacia complex (Bcc) are among the most important pathogens isolated from cystic fibrosis (CF) patients and in hospital acquired infections (HAI). Accurate identification of Bcc is questionable by conventional biochemical methods. Clonal typing of Burkholderia is also limited due to the problem with identification. Phenotypic identification methods such as VITEK2, protein signature identification methods like VITEK MS, Bruker Biotyper, and molecular targets such as 16S rRNA, recA, hisA and rpsU were reported with varying level of discrimination to identify Bcc. rpsU and/or 16S rRNA sequencing, VITEK2, VITEK MS and Bruker Biotyper could discriminate between Burkholderia spp. and non-Burkholderia spp. Whereas, Bcc complex level identification can be given by VITEK MS, Bruker Biotyper, and 16S rRNA/rpsU/recA/hisA sequencing. For species level identification within Bcc hisA or recA sequencing are reliable. Identification of Bcc is indispensable in CF patients and HAI to ensure appropriate antimicrobial therapy.
洋葱伯克霍尔德菌复合群（Bcc）中的细菌是从囊性纤维化（CF）患者和医院获得性感染（HAI）中分离出来的最重要的病原体之一。传统的生化方法对洋葱伯克霍尔德菌的鉴定存在疑问。由于鉴定问题，伯克霍尔德菌的克隆分型也受到限制。表型鉴定方法，如 VITEK2、蛋白质特征鉴定方法，如 VITEK MS、布鲁克生物梅里埃和分子靶标，如 16S rRNA、recA、hisA 和 rpsU，在识别 Bcc 方面具有不同的鉴别能力。rpsU 和/或 16S rRNA 测序、VITEK2、VITEK MS 和布鲁克生物梅里埃可以区分伯克霍尔德菌和非伯克霍尔德菌。然而，Bcc 复合体水平的鉴定可以通过 VITEK MS、布鲁克生物梅里埃和 16S rRNA/rpsU/recA/hisA 测序来完成。对于 Bcc 内的种水平鉴定，hisA 或 recA 测序是可靠的。在 CF 患者和 HAI 中，Bcc 的鉴定是必不可少的，以确保适当的抗菌治疗。
