Suzuki T, Muroga Y, Takahama M, Shiba T, Nishimura Y
Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:201-5. doi: 10.1099/00207713-49-1-201.
Phenotypic and phylogenetic studies were performed with two strains (OCh 317T and OCh 318; T = type strain) of aerobic chemoheterotrophic bacteriochlorophyll-containing bacteria isolated from water of a saline lake located on the west coast of Australia. Both strains were Gram-negative, short rods and were motile by means of polar flagella. Catalase, oxidase, nitrate reductase, phosphatase and urease were produced. The cells utilized D-glucose, citrate, glycolate, pyruvate and ethanol. Acids were produced from L-arabinose, D-fructose, D-galactose, D-glucose, D-ribose and D-xylose. The strains could grow in media containing 0.5-7.5% NaCl. Bacteriochlorophyll a was synthesized under aerobic conditions. The results of 16S rRNA gene sequence comparisons revealed that strain OCh 317T represented a new lineage in the alpha-3 group of the class Proteobacteria. Strains OCh 317T and OCh 318 were identified as strains of the same species because of their very similar phenotypic characteristics and their previously described high DNA-DNA homology. Therefore, it was concluded that the two strains should be assigned to a new genus and species, for which the name Rubrimonas cliftonensis is proposed. The type strain is OCh 317T (= JCM 10189T).
对从澳大利亚西海岸一个盐湖的水中分离出的两株需氧化学异养含细菌叶绿素的细菌（OCh 317T和OCh 318；T = 模式菌株）进行了表型和系统发育研究。两株菌均为革兰氏阴性短杆菌，通过极生鞭毛运动。它们能产生过氧化氢酶、氧化酶、硝酸还原酶、磷酸酶和脲酶。细胞利用D-葡萄糖、柠檬酸盐、乙醇酸、丙酮酸和乙醇。从L-阿拉伯糖、D-果糖、D-半乳糖、D-葡萄糖、D-核糖和D-木糖产生酸。这些菌株能在含有0.5 - 7.5%氯化钠的培养基中生长。细菌叶绿素a在有氧条件下合成。16S rRNA基因序列比较结果表明，菌株OCh 317T代表变形菌纲α-3组中的一个新谱系。由于菌株OCh 317T和OCh 318具有非常相似的表型特征以及先前描述的高DNA - DNA同源性，它们被鉴定为同一物种的菌株。因此，得出结论，这两株菌应归为一个新属和新种，为此提议命名为Cliftonensis红单胞菌。模式菌株是OCh 317T（= JCM 10189T）。
