Dale C, Maudlin I
Division of Molecular Genetics, University of Glasgow, UK.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:267-75. doi: 10.1099/00207713-49-1-267.
A secondary intracellular symbiotic bacterium was isolated from the haemolymph of the tsetse fly Glossina morsitans morsitans and cultured in Aedes albopictus cell line C6/36. Pure-culture isolation of this bacterium was achieved through the use of solid-phase culture under a microaerobic atmosphere. After isolation of strain M1T, a range of tests was performed to determine the phenotypic properties of this bacterium. Considering the results of these tests, along with the phylogenetic position of this micro-organism, it is proposed that this intracellular symbiont from G. m. morsitans should be classified in a new genus Sodalis gen. nov., as Sodalis glossinidius gen. nov., sp. nov. Strain M1T is the type strain for this new species.
从采采蝇(Glossina morsitans morsitans)的血淋巴中分离出一种次生细胞内共生细菌,并在白纹伊蚊细胞系C6/36中进行培养。通过在微需氧气氛下使用固相培养实现了该细菌的纯培养分离。分离出菌株M1T后,进行了一系列测试以确定该细菌的表型特性。考虑到这些测试结果以及该微生物的系统发育位置,建议将来自G. m. morsitans的这种细胞内共生体归类为一个新属—— Sodalis属(新属),命名为Sodalis glossinidius(新属,新种)。菌株M1T是该新物种的模式菌株。