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在低离子强度-硫酸鱼精蛋白自动分析仪系统中，通过使用范围扩展和更长的滤光波长改进抗体检测。

Improved antibody detection by the use of range expansion and longer filter wavelength in a low ionic strength-protamine sulphate Auto-Analyzer system.

作者信息

Downie D M, Voak D

出版信息

J Clin Pathol. 1976 Nov;29(11):991-4. doi: 10.1136/jcp.29.11.991.

DOI:10.1136/jcp.29.11.991
PMID:1002844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC476253/
Abstract

Range expansion, achieved by insertion of a variable resistance between the colorimeter and the recorder together with the use of 550 nm colorimeter filters, has resulted in markedly improved sensitivity for antibody detection, and improved sample identification, in a low ionic strength-protamine sulphate (LISPS) system. Range expansion also permits a lower concentration of red cells to be used, thus economizing on fully typed cells. Glycerol stored frozen cells were found to be only slightly less sensitive than fresh cells in this system.

摘要

通过在比色计和记录仪之间插入可变电阻以及使用550纳米比色计滤光片实现的量程扩展，已在低离子强度硫酸鱼精蛋白（LISPS）系统中显著提高了抗体检测的灵敏度，并改善了样本识别。量程扩展还允许使用较低浓度的红细胞，从而节省了完全分型的细胞。在该系统中，发现甘油冷冻保存的细胞仅比新鲜细胞的灵敏度略低。

相似文献

1
Improved antibody detection by the use of range expansion and longer filter wavelength in a low ionic strength-protamine sulphate Auto-Analyzer system.在低离子强度-硫酸鱼精蛋白自动分析仪系统中，通过使用范围扩展和更长的滤光波长改进抗体检测。
J Clin Pathol. 1976 Nov;29(11):991-4. doi: 10.1136/jcp.29.11.991.
2
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本文引用的文献

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A papain-bromelin-polybrene four-channel autoanalyzer system for blood group antibody screening. Analysis of 22,912 sera.用于血型抗体筛查的木瓜蛋白酶-菠萝蛋白酶-聚凝胺四通道自动分析仪系统。对22912份血清的分析。
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Quantitative haemagglutination. 6. Relationship of sialic acid content of red cells and aggregation by Polybrene, protamine and poly-L-lysine.
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A study of the characteristics of certain Rh antibodies preferentially detactable by enzyme technique.
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