硝基苯甲酰化后用液相色谱法测定饮料和食品中的糖醇
Liquid chromatographic determination of sugar alcohols in beverages and foods after nitrobenzoylation.
作者信息
Nojiri S, Saito K, Taguchi N, Oishi M, Maki T
机构信息
Tokyo Metropolitan Research Laboratory of Public Health, Tama Branch, Japan.
出版信息
J AOAC Int. 1999 Jan-Feb;82(1):134-40.
Use of p-nitrobenzoyl chloride (PNBC) to form an ultraviolet-absorbing derivative was attempted to determine the sugar alcohols meso-erythritol, xylitol, D-sorbitol, and D-mannitol by liquid chromatography (LC). LC determination of derivatives was performed on an ODS column with acetonitrile-water (65 + 35) as mobile phase. Calibration curves were linear in the concentration range 0.01-100 micrograms/mL. Method sensitivity is 10 to 1000 times higher than that of LC with refractive index detection and gas chromatography with flame ionization detection. Recoveries of sugars added to various foods at 0.1 and 1% ranged from 91 to 102% for meso-erythritol, 75 to 115% for xylitol, 81 to 105% for D-sorbitol, and 81 to 108% for D-mannitol.
尝试使用对硝基苯甲酰氯（PNBC）形成一种紫外线吸收衍生物，通过液相色谱法（LC）测定糖醇内消旋赤藓醇、木糖醇、D - 山梨醇和D - 甘露糖醇。在以乙腈 - 水（65 + 35）为流动相的ODS柱上进行衍生物的LC测定。校准曲线在0.01 - 100微克/毫升的浓度范围内呈线性。该方法的灵敏度比用折光率检测的LC和用火焰离子化检测的气相色谱法高10至1000倍。添加到各种食品中浓度为0.1%和1%的糖的回收率，内消旋赤藓醇为91%至102%，木糖醇为75%至115%，D - 山梨醇为81%至105%，D - 甘露糖醇为81%至108%。
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