Asakura S, Kato H, Fujino S, Konishi T, Tezuka N, Mori A
Second Department of Surgery, Shiga University of Medical Science, Japan.
Hum Pathol. 1999 Feb;30(2):195-8. doi: 10.1016/s0046-8177(99)90275-7.
Transforming growth factor-beta1 (TGF-beta1) is known as the growth factor that stimulates the synthesis of extracellular matrix. Recently, TGF-beta has been found to control the growth of cancer cells. Small chondroitin-dermatan sulfate (decorin) is an abundant extracellular matrix component. TGF-beta1 stimulates the synthesis of decorin, and decorin is considered to bind TGF-beta1. The activity of decorin in neutralizing TGF-beta1 activity suggests that decorin serves as a negative-feedback regulator of TGF-beta1 activity. To investigate the role and relationship of TGF-beta1 and decorin in the formation of central fibrosis in pulmonary adenocarcinoma, we performed an immunohistochemical study of TGF-beta1 and decorin in 61 cases of T1 pulmonary adenocarcinoma. Positive stainings for TGF-beta1 were shown in 40 cases and negative in 21 cases. Twenty-seven of 32 cases with central fibrosis were positive for TGF-beta1. Positive staining for TGF-beta1 was significantly related to the appearance of central fibrosis in pulmonary adenocarcinoma. When central fibrosis was composed of proliferative connective tissue with loose staining for decorin, cancer cells showed intense staining for TGF-beta1. When central fibrosis was composed of old fibrotic tissue with dense staining for decorin, cancer cells showed weak staining for TGF-beta1. Our results suggest that TGF-beta1 has an important role in the formation of central fibrosis in pulmonary adenocarcinoma, and decorin may play a role as a negative feedback regulator in the production of TGF-beta1 in pulmonary adenocarcinoma.
转化生长因子-β1（TGF-β1）是一种已知能刺激细胞外基质合成的生长因子。最近，人们发现TGF-β可控制癌细胞的生长。小分子硫酸软骨素-硫酸皮肤素（核心蛋白聚糖）是一种丰富的细胞外基质成分。TGF-β1刺激核心蛋白聚糖的合成，并且核心蛋白聚糖被认为可与TGF-β1结合。核心蛋白聚糖在中和TGF-β1活性方面的作用表明，核心蛋白聚糖充当TGF-β1活性的负反馈调节因子。为了研究TGF-β1和核心蛋白聚糖在肺腺癌中央纤维化形成中的作用及关系，我们对61例T1期肺腺癌患者的TGF-β1和核心蛋白聚糖进行了免疫组织化学研究。TGF-β1阳性染色40例，阴性21例。32例有中央纤维化的病例中，27例TGF-β1呈阳性。TGF-β1阳性染色与肺腺癌中央纤维化的出现显著相关。当中央纤维化由对核心蛋白聚糖染色疏松的增殖性结缔组织组成时，癌细胞显示TGF-β1强染色。当中央纤维化由对核心蛋白聚糖染色致密的陈旧性纤维化组织组成时，癌细胞显示TGF-β1弱染色。我们的结果表明，TGF-β1在肺腺癌中央纤维化的形成中起重要作用，并且核心蛋白聚糖可能在肺腺癌中TGF-β1的产生中作为负反馈调节因子发挥作用。
