Dogra S, Krishnamurthy S, Gupta V, Dixit B L, Gupta C M, Sanglard D, Prasad R
Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Yeast. 1999 Jan 30;15(2):111-21. doi: 10.1002/(SICI)1097-0061(19990130)15:2<111::AID-YEA350>3.0.CO;2-E.
By using two molecular probes, we demonstrate that only 4% of total phosphatidylethanolamine (PtdEtn) in the plasma membrane (PM) of a human pathogenic yeast, Candida albicans, is present in its external half. Evidence is presented to show that the availability of PtdEtn could be related to the expression of a multidrug transporter CDR1 of C. albicans, and the process is energy-dependent. A homozygous CDR1 disruptant strain of C. albicans shows almost 23% reduction in the external labelling of PtdEtn. This report shows that, similar to human MDRs, yeast multidrug transporter could also be involved in aminophospholipid translocation.
通过使用两种分子探针，我们证明在人类致病酵母白色念珠菌的质膜（PM）中，仅4%的总磷脂酰乙醇胺（PtdEtn）存在于其外半层。有证据表明，PtdEtn的可用性可能与白色念珠菌多药转运蛋白CDR1的表达有关，且该过程依赖能量。白色念珠菌的纯合CDR1缺失菌株在PtdEtn的外部标记上显示出近23%的减少。本报告表明，与人类多药耐药蛋白类似，酵母多药转运蛋白也可能参与氨基磷脂的转运。
