Perez-Lorenzo S, Levy-Benshimol A, Gomez-Acevedo S
Centro de Biología Celular, Facultad de Ciencias, U.C.V., Caracas, Venezuela.
Acta Cient Venez. 1998;49(3):144-51.
The presence of lectins, tannins and protease inhibitors was studied in 27 algae species collected at four Venezuelan coral rift sites. Among the species studied, only six had hemagglutinating activity, apparently due to their lectin content. Higher hemagglutinating titers were obtained when the extracts were tested on pronase-treated erythrocytes. Hemagglutination was inhibited by simple sugars and by bovine submaxillary gland mucine. GaINAc was the only inhibitor of the hemagglutination caused by Grateulopia filicina extracts. None of the compounds tested inhibited the hemagglutination caused by Halimeda opuntia. The polyvinylpolypirrolidone treatment abolished the hemagglutinating activity of both brown and red algae. However, in Grateulopia filicina and Hypnea cervicornis (Rhodophyta) hemagglutinating activity persisted after the polyvinylpolypirrolidone treatment, presumably due to the presence of true lectins in those algae. Tannin content (presumably phlorotannins) was higher in the Phaeophyta as compared to the Rhodophyta. The brown alga Padina gymnospora had the higher content of these polyphenols. Trypsin inhibitors were detected, in minute ammounts, only in Padina gymnospora (Phaeophyta) and Acantophora spicifera (Rhodophyta). No subtilisin inhibition was observed whatsoever.
对在委内瑞拉四个珊瑚礁地点采集的27种藻类进行了凝集素、单宁和蛋白酶抑制剂的研究。在所研究的物种中，只有六种具有血凝活性，显然是由于其凝集素含量。当提取物在经链霉蛋白酶处理的红细胞上进行测试时，获得了更高的血凝滴度。血凝反应受到单糖和牛颌下腺粘蛋白的抑制。N-乙酰半乳糖胺是由丝状葡萄藻提取物引起的血凝反应的唯一抑制剂。所测试的化合物均未抑制仙人掌藻引起的血凝反应。聚乙烯聚吡咯烷酮处理消除了褐藻和红藻的血凝活性。然而，在丝状葡萄藻和角叉菜（红藻门）中，聚乙烯聚吡咯烷酮处理后血凝活性仍然存在，推测是由于这些藻类中存在真正的凝集素。与红藻相比，褐藻中的单宁含量（可能是褐藻多酚）更高。褐藻裸叶藻的这些多酚含量最高。仅在裸叶藻（褐藻门）和尖刺顶群藻（红藻门）中检测到微量的胰蛋白酶抑制剂。未观察到对枯草杆菌蛋白酶的抑制作用。
