Kovi J, Morris H P
J Natl Cancer Inst. 1976 Jul;57(1):197-205. doi: 10.1093/jnci/57.1.197.
A pituitary tumor was induced in a female inbred BUF rat on an 18-month diet containing 1.1 mmole 2,4,6-trimethylaniline/kg. In the sixth transfer, this tumor developed into two lines of transplantable tumors with different characteristics. Here these two lines were studied by light and electron microscopy; histologically, both tumors were well-differentiated pituitary carcinomas. In the ultrathin sections, the neoplastic cells were separated by a wide intercellular space and covered by numerous microvilli. In the mammosomatotrophic tumor (7315a) the neoplastic cells contained big, electron-dense, ovoid or globular secretory granules (560-1,700 nm in size) that were similar to the prolactin granules of mammotrophs. However, in these cells were also small, pale, uniform, secretory granules that were along the plasma membrane, measured 160 nm in diameter, and resembled the ACTH-containing granules of corticotrophs. The neoplastic cells in tumor line 7315i possessed secretory granules comparable to the granules of the ACTH-secreting cells. The differentiation of the ergastoplasm was abnormal. The tumor cells contained an endoplasmic reticulum similar to mammotrophs and somatotrophs but dissimilar to ACTH-secreting cells. These investigations suggested that the production of several hormones in transplantable pituitary tumors resulted from the multisecretory differentiation of one neoplastic pituitary cell.
给一只雌性近交BUF大鼠喂食含1.1毫摩尔/千克2,4,6-三甲基苯胺的饲料18个月后诱发了垂体肿瘤。在第六次传代时，该肿瘤发展成具有不同特征的两株可移植肿瘤。在此对这两株肿瘤进行了光镜和电镜研究；组织学上，两种肿瘤均为高分化垂体癌。在超薄切片中，肿瘤细胞被宽阔的细胞间隙分隔，表面有许多微绒毛。在促乳腺生长激素瘤（7315a）中，肿瘤细胞含有大的、电子致密的、卵圆形或球形分泌颗粒（大小为560 - 1700纳米），类似于促乳腺细胞的催乳素颗粒。然而，在这些细胞中也有小的、浅色的、均匀的分泌颗粒，沿质膜分布，直径为160纳米，类似于促肾上腺皮质激素细胞的含促肾上腺皮质激素颗粒。7315i肿瘤株中的肿瘤细胞具有与促肾上腺皮质激素分泌细胞颗粒相当的分泌颗粒。粗面内质网的分化异常。肿瘤细胞含有与促乳腺细胞和生长激素细胞相似但与促肾上腺皮质激素分泌细胞不同的内质网。这些研究表明，可移植垂体肿瘤中多种激素的产生是由一个肿瘤性垂体细胞的多分泌分化所致。
