Mahagaokar S, Rao P N, Barlogie B
J Natl Cancer Inst. 1976 Dec;57(6):1305-10. doi: 10.1093/jnci/57.6.1305.
We sutdied the effect of 3,6-bis(5-chloro-2-piperidinyl)-2,5-piperazinedione (BCP) on various parameters of the replication cycle and cell grwoth on monolayer cultures of Chinese hamster ovary cells. The results indicated that BCP had no effect on the G-S transition or the passage of cells from metaphase to G. However, BCP prolonged S- and G-phases because of its retarding effect on DNA synthetis. BCP was more toxic to S-phase cells than to G or mitotic cells. As with other alkylating agents, chromosome damage due to BCP could be seen only in cells that had undergone DNA replication after exposure to the drug.
我们研究了3,6 - 双（5 - 氯 - 2 - 哌啶基）- 2,5 - 哌嗪二酮（BCP）对中国仓鼠卵巢细胞单层培养物复制周期和细胞生长的各种参数的影响。结果表明，BCP对G - S转换或细胞从中期到G期的进程没有影响。然而，由于其对DNA合成的阻滞作用，BCP延长了S期和G期。BCP对S期细胞的毒性比对G期或有丝分裂期细胞更大。与其他烷化剂一样，只有在接触该药物后经历了DNA复制的细胞中才能看到BCP引起的染色体损伤。
