Hirose N, Yoshida Y, Koide S, Takada K, Saigusa T, Koshikawa N
Department of Dental Anesthesiology, Nihon University School of Dentistry, Tokyo, Japan.
J Oral Sci. 1998 Dec;40(4):165-70. doi: 10.2334/josnusd.40.165.
The effects of propofol, a hydrophobic intravenous anaesthetic, and fentanyl, an opiate analgesic, on extracellular concentrations of gamma-aminobutyric acid (GABA) in the nucleus accumbens of rats were studied using in vivo brain microdialysis. The concentrations of GABA in the microdialysate of the nucleus accumbens reached a stable baseline value approximately 24 h after probe insertion and were not affected over the ensuing 250 min by intravenous injection of vehicle (1.0 ml/kg) or perfusion of tetrodotoxin (2 microM) into the nucleus accumbens via the dialysis membrane. Propofol (2.5 mg/kg and 10.0 mg/kg i.v.) did not significantly affect the accumbens microdialysate concentration of GABA over the 250 min of quantification. Fentanyl (100 microM) infused for 25 min into the nucleus accumbens via the dialysis membrane produced a marked and transient increase in accumbens GABA concentration to a maximum of approximately 1200% at its peak effect that occurred at 25 min after cessation of the fentanyl infusion.
使用体内脑微透析技术，研究了疏水性静脉麻醉剂丙泊酚和阿片类镇痛药芬太尼对大鼠伏隔核细胞外γ-氨基丁酸（GABA）浓度的影响。在探针插入后约24小时，伏隔核微透析液中GABA的浓度达到稳定的基线值，在随后的250分钟内，静脉注射溶媒（1.0 ml/kg）或通过透析膜向伏隔核灌注河豚毒素（2 μM）均未对其产生影响。在250分钟的定量过程中，丙泊酚（静脉注射2.5 mg/kg和10.0 mg/kg）对伏隔核微透析液中GABA的浓度没有显著影响。通过透析膜向伏隔核内注入芬太尼（100 μM）25分钟，可使伏隔核GABA浓度显著且短暂升高，在芬太尼输注停止后25分钟达到峰值效应时，最高可达约1200%。
