Heidbreder C, De Witte P
Laboratoire de Psychobiologie, Croix du Sud, Louvain-la-Neuve, Belgium.
Pharmacol Biochem Behav. 1993 Oct;46(2):477-81. doi: 10.1016/0091-3057(93)90383-5.
The magnitude, direction, and time course of the effects of acute administration of ethanol (0.1 or 1.0 g/kg) on the extracellular levels of dopamine (DA), dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), and GABA within the nucleus accumbens of the rat were analysed using microdialysis in conjunction with high-pressure liquid chromatography and electrochemical detection. IP injection of 0.1 g/kg ethanol failed to modify the extracellular concentration of DA, DOPAC, HVA, 5-HT, 5-HIAA, and GABA in the nucleus accumbens during the 120-min collection period. On the contrary, 1.0 g/kg IP ethanol significantly increased extracellular levels of DA, DOPAC, and 5-HIAA in microdialysates from the nucleus accumbens. The maximal increase in DA (314.87 +/- 6%), DOPAC (210.58 +/- 3%), and 5-HIAA (250.88 +/- 8%) was observed 40 min after administration of ethanol. Extracellular HVA was also enhanced (220.89 +/- 2%) at time point 100 min following injection of the same dose of ethanol. Finally, extracellular levels of 5-HT and GABA remained unchanged following 1.0 g/kg ethanol.
采用微透析结合高压液相色谱和电化学检测法，分析了急性给予乙醇（0.1或1.0 g/kg）对大鼠伏隔核细胞外多巴胺（DA）、二羟基苯乙酸（DOPAC）、高香草酸（HVA）、5-羟色胺（5-HT）、5-羟吲哚乙酸（5-HIAA）和γ-氨基丁酸（GABA）水平的影响程度、方向和时间进程。腹腔注射0.1 g/kg乙醇在120分钟的采集期内未改变伏隔核中DA、DOPAC、HVA、5-HT、5-HIAA和GABA的细胞外浓度。相反，腹腔注射1.0 g/kg乙醇显著增加了伏隔核微透析液中DA、DOPAC和5-HIAA的细胞外水平。给予乙醇40分钟后，DA（314.87±6%）、DOPAC（210.58±3%）和5-HIAA（250.88±8%）的增加达到最大值。注射相同剂量乙醇100分钟时，细胞外HVA也有所升高（220.89±2%）。最后，给予1.0 g/kg乙醇后，5-HT和GABA细胞外水平保持不变。
