Boudreau-Larivière C, Jasmin B J
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ont., Canada.
FEBS Lett. 1999 Feb 5;444(1):22-6. doi: 10.1016/s0014-5793(99)00015-0.
Nerve-derived trophic factors are known to modulate expression of acetylcholinesterase (AChE) in skeletal muscle fibers, yet the precise identity of these factors remains elusive. In the present study, we treated mouse C2 myotubes with calcitonin gene-related peptide (CGRP). Compared to non-treated myotubes, cell-associated AChE activity levels were decreased by approximately 60% after 48 h of treatment. A parallel reduction in AChE total protein levels was also observed as determined by Western blot analysis. The reduction in AChE activity was due to a decrease in the levels of the G1 molecular form and to an elimination of G1. By contrast, levels of secreted AChE remained unchanged following CGRP treatment. Finally, the overall decrease in AChE activity was accompanied by a reduction in AChE transcripts which could not be attributed to changes in the transcriptional rate of the ACHE gene.
已知神经源性营养因子可调节骨骼肌纤维中乙酰胆碱酯酶（AChE）的表达，然而这些因子的确切身份仍然难以捉摸。在本研究中，我们用降钙素基因相关肽（CGRP）处理小鼠C2肌管。与未处理的肌管相比，处理48小时后，细胞相关的AChE活性水平降低了约60%。通过蛋白质印迹分析还观察到AChE总蛋白水平平行下降。AChE活性的降低是由于G1分子形式水平的降低以及G1的消除。相比之下，CGRP处理后分泌型AChE的水平保持不变。最后，AChE活性的总体下降伴随着AChE转录本的减少，这不能归因于ACHE基因转录速率的变化。
