Choi R C, Leung P W, Dong T T, Wan D C, Tsim K W
Department of Biology, Hong Kong University of Science and Technology, Kowloon, Hong Kong.
Neurosci Lett. 1996 Oct 18;217(2-3):165-8.
Calcitonin gene-related peptide (CGRP), a neuropeptide may play a role in the formation of neuromuscular junctions is synthesized by the motor neurons and is able to stimulate the expression of acetylcholine receptor (AChR) in cultured myotubes. By using antibody and DNA probe that are specific for acetyl cholinesterase (AChE), we reported the expression of AChE could also be stimulated by CGRP in cultured chick myotubes. After CGRP application, the amount of AChE protein, that showed a molecular weight of approximately 105 kDa as recognized by the anti-AChE monoclonal antibody, was increased by approximately 1.7-fold. Two transcripts encoding AChE, approximately 4.8 and approximately 6.0 kb, were identified and their levels of expression were increased to approximately 3-fold after treatment with CGRP. However, the total AChE enzymatic activity in the CGRP-treated myotubes was unchanged. These evidences suggest that most of the CGRP-induced AChE proteins in the cultured chick myotubes are the inactive pool of enzyme.
降钙素基因相关肽（CGRP）是一种神经肽，由运动神经元合成，可能在神经肌肉接头的形成中发挥作用，并且能够刺激培养的肌管中乙酰胆碱受体（AChR）的表达。通过使用针对乙酰胆碱酯酶（AChE）的特异性抗体和DNA探针，我们报道了在培养的鸡肌管中，AChE的表达也可被CGRP刺激。应用CGRP后，抗AChE单克隆抗体识别的分子量约为105 kDa的AChE蛋白量增加了约1.7倍。鉴定出了编码AChE的两种转录本，大小约为4.8 kb和约6.0 kb，用CGRP处理后它们的表达水平增加到约3倍。然而，CGRP处理的肌管中总的AChE酶活性没有变化。这些证据表明，培养的鸡肌管中大多数CGRP诱导的AChE蛋白是酶的无活性库。
