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Direct observation of DNA chain orientation and relaxation by electric birefringence: Implications for the mechanism of separation during pulsed-field gel electrophoresis.

作者信息

Sturm J, Weill G

出版信息

Phys Rev Lett. 1989 Mar 27;62(13):1484-1487. doi: 10.1103/PhysRevLett.62.1484.

DOI:10.1103/PhysRevLett.62.1484
PMID:10039686
Abstract
摘要

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Direct observation of DNA chain orientation and relaxation by electric birefringence: Implications for the mechanism of separation during pulsed-field gel electrophoresis.通过电场双折射直接观察DNA链的取向和松弛:对脉冲场凝胶电泳分离机制的启示
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Transient electric birefringence of agarose gels. I. Unidirectional electric fields.琼脂糖凝胶的瞬态电双折射。I. 单向电场。
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Numerical simulation of gel electrophoresis of DNA knots in weak and strong electric fields.弱电场和强电场中DNA纽结凝胶电泳的数值模拟
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Change of the higher order structure of DNA induced by the complexation with intercalating synthetic polymer, as is visualized by fluorescence microscopy.通过荧光显微镜观察到,与插入型合成聚合物络合会导致DNA高阶结构发生变化。
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