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[链霉菌属放线菌中经煮沸处理的关节孢子活力修复]

[Reparation of viability of arthrospores subjected to boiling in Actinomyces streptomycini].

作者信息

Lapteva E A, Agre N S, Kalakutskiĭ L V

出版信息

Mikrobiologiia. 1976 May-Jun;45:559-61.

PMID:1004259
Abstract

Endospores of Streptomyces streptomycini B-16 are considerably more thermostable (by 40degreesC) than it was generally accepted. The bulk of populations of the spores (up to 55%) can be subjected to 100degreesC during 10 min but does not germinate under usual conditions as a result of heat damage which may be repaired at a relative humidity of 100% or in water suspensions at temperatures optimal for growth of the actinomycete. The rate of reparation increase upon washing of the heated spores with stirring, centrifuging and especially after treating the spores with ultrasound. The treatment is supposed to eliminate from the spores growth inhibitors which were formed as a result of heat damage. Characteristics of the process are discussed, which make it different from other reparation systems described elsewhere, in particular its endogenous character.

摘要

链霉素链霉菌B-16的内生孢子的耐热性比普遍认为的要高得多(高40摄氏度)。大部分孢子群体(高达55%)在100摄氏度下处理10分钟,但在通常条件下不会萌发,这是由于热损伤所致,而这种损伤在相对湿度为100%或在放线菌生长最佳温度的水悬浮液中可得到修复。在搅拌、离心洗涤加热后的孢子,尤其是用超声波处理孢子后,修复速率会提高。该处理被认为可从孢子中消除因热损伤而形成的生长抑制剂。文中讨论了该过程的特点,这些特点使其有别于其他地方描述的修复系统,尤其是其内生特性。

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