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利用细胞质提取物对RNA进行加工。将分离的HeLa细胞核与细胞质提取物一起孵育，对RNA进行合成和加工。

Processing of RNA by cytoplasmic extract. Synthesis and processing of RNA by isolated HeLa cell nuclei incubated with cytoplasmic extract.

作者信息

Sarma M H

出版信息

Mol Biol Rep. 1976 Sep;3(1):47-54. doi: 10.1007/BF00357208.

DOI:10.1007/BF00357208
PMID:1004505
Abstract

Nuclei are isolated from HeLa cells by a low speed centrifugation procedure. These nuclei can carry out RNA synthesis at 30 degrees. The effect of cytoplasmic extract on the transport and processing of RNA is measured. In the presence of cytoplasmic extract the precursor to 4S RNA is processed to 4S RNA and an RNA species OF 7-9S is transported outside the nuclei.

摘要

通过低速离心程序从HeLa细胞中分离出细胞核。这些细胞核能在30摄氏度下进行RNA合成。测定细胞质提取物对RNA转运和加工的影响。在细胞质提取物存在的情况下，4S RNA的前体被加工成4S RNA，一种7 - 9S的RNA种类被转运到细胞核外。

相似文献

1
Processing of RNA by cytoplasmic extract. Synthesis and processing of RNA by isolated HeLa cell nuclei incubated with cytoplasmic extract.利用细胞质提取物对RNA进行加工。将分离的HeLa细胞核与细胞质提取物一起孵育，对RNA进行合成和加工。
Mol Biol Rep. 1976 Sep;3(1):47-54. doi: 10.1007/BF00357208.
2
Inhibitory effects of dialyzed leukocyte extract on DNA and RNA synthesis.透析白细胞提取物对DNA和RNA合成的抑制作用。
Basic Appl Histochem. 1987;31(3):325-30.
3
RNA synthesis in isolated HeLa cell nuclei.
Biochim Biophys Acta. 1976 Jan 5;418(1):29-38. doi: 10.1016/0005-2787(76)90324-5.
4
The metabolism of heterogeneous nuclear RNA and the formation of cytoplasmic messenger RNA in animal cells.动物细胞中核不均一RNA的代谢及细胞质信使RNA的形成。
Prog Biophys Mol Biol. 1976;31(1):1-38. doi: 10.1016/0079-6107(78)90003-2.
5
Rna synthesis in isolated nuclei processing of adenovirus serotype 2 late messenger rna precursors.2型腺病毒晚期信使核糖核酸前体在分离细胞核中的核糖核酸合成。
J Mol Biol. 1982 Aug 25;159(4):581-99. doi: 10.1016/0022-2836(82)90102-4.
6
RNA metabolism in nuclei: selective transport of kappa exons from myeloma nuclei and adenoviral transcripts from infected HeLa nuclei.细胞核中的RNA代谢：骨髓瘤细胞核中κ外显子的选择性转运以及感染的HeLa细胞核中腺病毒转录本的选择性转运。
Nucleic Acids Res. 1986 May 27;14(10):4159-69. doi: 10.1093/nar/14.10.4159.
7
In vitro synthesis of a 5S RNA precursor by isolated nuclei of rat liver and HeLa cells.
Cell. 1979 May;17(1):163-73. doi: 10.1016/0092-8674(79)90304-0.
8
Reinitiation of synthesis of small cytoplasmic RNA species K and L in isolated HeLa cell nuclei in vitro.体外分离的HeLa细胞核中小细胞质RNA种类K和L合成的重新起始
Nucleic Acids Res. 1980 Jan 25;8(2):225-34. doi: 10.1093/nar/8.2.225.
9
Essential factors in the kinetic analysis of RNA synthesis in HeLa cells.HeLa细胞中RNA合成动力学分析的关键因素。
J Cell Physiol. 1977 Mar;90(3):521-34. doi: 10.1002/jcp.1040900315.
10
[Direct and reverse transport of RNA in a system of isolated HeLa cell nuclei].[RNA在分离的HeLa细胞核系统中的正向和逆向转运]
Biokhimiia. 1984 Dec;49(12):1955-8.

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Further evidence of transcriptional and translational control of histone messenger RNA during the HeLa S3 cycle.海拉S3细胞周期中组蛋白信使核糖核酸转录与翻译控制的进一步证据。
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