Sonina N V, Shuppe N G
Biokhimiia. 1984 Dec;49(12):1955-8.
In a system containing isolated HeLa cell nuclei the release of RNA from the nuclei may be paralleled with the antagonistic process, i. e., RNA translocation into the nuclei. The RNA release from the nuclei depends on incubation time, pH, Mg2+ and nucleoside triphosphate concentration. The rate of reverse transport depends on pH, size of RNA to be translocated and the physiological state of the nuclear membrane. Low molecular weight RNAs (less than 10 S) are translocated into the nuclei most effectively. The nuclei of synchronized HeLa cells in the G1-period are more "permeable" for translocated RNA as compared with the S-phase HeLa cell nuclei.
在一个包含分离的海拉细胞核的系统中，细胞核中RNA的释放可能与拮抗过程，即RNA向细胞核内的转运同时发生。细胞核中RNA的释放取决于孵育时间、pH值、镁离子和三磷酸核苷浓度。逆向转运的速率取决于pH值、待转运RNA的大小以及核膜的生理状态。低分子量RNA（小于10S）最有效地转运到细胞核中。与S期海拉细胞核相比，处于G1期的同步化海拉细胞核对转运进来的RNA更“通透”。
