Nakopoulou L, Stefanaki K, Vourlakou C, Manolaki N, Gakiopoulou H, Michalopoulos G
Dept. of Pathology, Medical School Univ. of Athens, Greece.
Pathol Res Pract. 1999;195(1):19-24. doi: 10.1016/S0344-0338(99)80089-2.
Bcl-2 protein blocks apoptosis and is involved in human intrahepatic bile-duct development. Formalin-fixed, paraffin-embedded archival tissue from 42 HBV and HCV hepatitis [20 acute AH, 22 chronic hepatitis (CH)], 12 active cirrhosis (CR) and 20 hepatocellular carcinoma (HCC) was immunostained for bcl-2 protein. In all cases, bcl-2 protein was detected in portal and intralobular lymphocytes but not in hepatocytes or Kupffer cells. Bcl-2 was positive in the cytoplasm of small portal bile ducts of chronic hepatitis, while it was strongly expressed in newly formed bile-ductules of the limiting plate, mainly in CH with marked activity and CR. Bcl-2 was detected in small bile ducts in only one case of acute hepatitis and was not detected in any case of HCC. Bcl-2 seems to be involved in the regulation of growth and apoptosis of cholangiolar cells. Its expression in small bile ducts and in newly-formed ductules especially in CH with marked activity and CR, implies that the embryonic model of intrahepatic bile duct development may be recapitulated in chronic hepatic disease. Moreover, it supports evidence for the existence of the controversial long-lived stem population in the liver. Bcl-2 does not seem to be involved in hepatocarcinogenesis.
Bcl-2蛋白可阻止细胞凋亡，并参与人类肝内胆管的发育。对42例乙肝和丙肝肝炎患者（20例急性肝炎、22例慢性肝炎）、12例活动性肝硬化及20例肝细胞癌的福尔马林固定、石蜡包埋存档组织进行Bcl-2蛋白免疫染色。在所有病例中，Bcl-2蛋白在门脉区和小叶内淋巴细胞中被检测到，但在肝细胞或库普弗细胞中未检测到。Bcl-2在慢性肝炎的小门静脉胆管细胞质中呈阳性，而在界板新形成的胆小管中强烈表达，主要见于活动明显的慢性肝炎和肝硬化。仅在1例急性肝炎病例的小胆管中检测到Bcl-2，在任何肝细胞癌病例中均未检测到。Bcl-2似乎参与胆管细胞生长和凋亡的调节。其在小胆管和新形成的胆小管中的表达，尤其是在活动明显的慢性肝炎和肝硬化中的表达，意味着肝内胆管发育的胚胎模型可能在慢性肝病中重现。此外，这支持了肝脏中存在有争议的长寿干细胞群体的证据。Bcl-2似乎不参与肝癌的发生。
