Escarpa A, González M C
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá, Madrid, Spain.
J Chromatogr A. 1999 Jan 15;830(2):301-9. doi: 10.1016/s0021-9673(98)00893-0.
Polyphenolic compounds in apples and pears were analysed by HPLC on C18-modified silica. Gradient elution with phosphoric acid-methanol mixtures and phosphoric acid-acetonitrile mixtures gave complete separation of all polyphenolics of interest. The use of methanol as modifier was preferred because it provides a more rapid separation (20 min). Diode-array detection was used for the provisional identification of polyphenolic compounds not available as standards.
采用高效液相色谱法在C18改性硅胶柱上分析苹果和梨中的多酚类化合物。用磷酸-甲醇混合物和磷酸-乙腈混合物进行梯度洗脱，可使所有目标多酚类化合物完全分离。选用甲醇作为改性剂，因为它能实现更快的分离（20分钟）。对于没有标准品的多酚类化合物，采用二极管阵列检测进行初步鉴定。
