Al-Aswad L A, Huang M, Netland P A
Department of Ophthalmology, University of Tennessee, Memphis 38163, USA.
J Ocul Pharmacol Ther. 1999 Feb;15(1):41-9. doi: 10.1089/jop.1999.15.41.
Our purpose was to study the antiproliferative effect of cytosine arabinoside (Ara-C) on rabbit Tenon's fibroblasts and the efficacy of Ara-C as an adjunctive antifibrosis treatment for glaucoma filtration surgery in the rabbit eye. Rabbit Tenon's fibroblasts were exposed to 1 microg/ml of Ara-C for various time intervals, then cell number and viability was assessed at different time points. Following posterior lip sclerectomy, rabbit eyes were treated with 10 mg subconjunctival Ara-C daily for 7 days, then every other day for 7 days. Rabbit fibroblasts exposed to 1 microg/ml Ara-C for 1 hour showed no significant decrease in cell number compared with control. Continuous or 24-hour incubation of fibroblasts with Ara-C was lethal to the cells. Exposure of cells to Ara-C for 3-, 6-, and 9-hour intervals caused significant reduction of cell proliferation. Pulsed treatment of cells with 6 hour exposure to 1 microg/ml Ara-C every 3 days caused prolonged suppression of cell proliferation. Following posterior lip sclerectomy in rabbits, topical instillation of Ara-C drops at varying concentrations and dosing intervals did not cause any significant lowering of intraocular pressure compared with control eyes, although bleb survival was prolonged in eyes treated with Ara-C (P < 0.01). In rabbit eyes treated postoperatively with subconjunctival injections of 10 mg Ara-C every other day for two weeks, the mean intraocular pressure was significantly decreased and the bleb survival time was significantly prolonged (P < 0.0067) compared with control eyes. In conclusion, Ara-C inhibits rabbit Tenon's fibroblast proliferation in vitro. Postoperative subconjunctival injection of Ara-C results in improved bleb function after filtration surgery in the rabbit.
我们的目的是研究阿糖胞苷（Ara-C）对兔眼Tenon囊成纤维细胞的抗增殖作用，以及Ara-C作为兔眼青光眼滤过手术辅助抗纤维化治疗的疗效。将兔Tenon囊成纤维细胞暴露于1微克/毫升的Ara-C中不同时间间隔，然后在不同时间点评估细胞数量和活力。在后唇巩膜切除术后，兔眼每天结膜下注射10毫克Ara-C，共7天，然后隔天注射7天。与对照组相比，暴露于1微克/毫升Ara-C 1小时的兔成纤维细胞数量没有显著减少。成纤维细胞与Ara-C连续或24小时孵育对细胞具有致死性。细胞暴露于Ara-C 3小时、6小时和9小时会导致细胞增殖显著减少。每3天用1微克/毫升Ara-C暴露细胞6小时的脉冲处理导致细胞增殖的长期抑制。在兔后唇巩膜切除术后，与对照眼相比，局部滴注不同浓度和给药间隔的Ara-C滴眼液不会导致眼压显著降低，尽管用Ara-C治疗的眼的滤过泡存活时间延长（P<0.01）。在兔眼术后每隔一天结膜下注射10毫克Ara-C两周，与对照眼相比，平均眼压显著降低，滤过泡存活时间显著延长（P<0.0067）。总之，Ara-C在体外抑制兔Tenon囊成纤维细胞增殖。术后结膜下注射Ara-C可改善兔滤过手术后的滤过泡功能。
