Miyamoto K, Tatsumi S, Segawa H, Morita K, Nii T, Fujioka A, Kitano M, Inoue Y, Takeda E
Department of Clinical Nutrition, School of Medicine, Tokushima University, Tokushima City, Japan.
Nephrol Dial Transplant. 1999;14 Suppl 1:73-5. doi: 10.1093/ndt/14.suppl_1.73.
A cDNA encoding an Na+-Pi co-transporter, termed rat PiT-1, has now been isolated from rat parathyroid. Expression of rat PiT-1 in Xenopus oocytes revealed that it possesses Na+-dependent Pi co-transport activity. The amount of PiT-1 mRNA in the parathyroid of vitamin D-deficient rats was reduced compared with that in normal animals, and increased markedly after administration of 1,25-dihydroxyvitamin D3. Furthermore, the abundance of PiT-1 mRNA in the parathyroid was much greater in rats fed a low-Pi diet than in those fed a high-Pi diet. Thus, rat PiT-1 may contribute to the effects of Pi and vitamin D on parathyroid function.
一种编码钠-无机磷共转运体的互补DNA（cDNA），被命名为大鼠PiT-1，现已从大鼠甲状旁腺中分离出来。大鼠PiT-1在非洲爪蟾卵母细胞中的表达表明，它具有钠依赖性无机磷共转运活性。与正常动物相比，维生素D缺乏大鼠甲状旁腺中PiT-1 mRNA的量减少，而在给予1,25-二羟基维生素D3后显著增加。此外，低磷饮食喂养的大鼠甲状旁腺中PiT-1 mRNA的丰度远高于高磷饮食喂养的大鼠。因此，大鼠PiT-1可能参与了无机磷和维生素D对甲状旁腺功能的影响。
